Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4 (Phospho-Ser245) Antibody (left) or the same antibody preincubated with blocking peptide (right)| Product Name | ATF4 (phospho Ser245) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATF4 (Phospho Ser245)ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication. |
| Immunogen | Peptide sequence around phosphorylation site of serine 245 (N-R-S(p)-L-P) derived from Human ATF4. |
| Modification | p-Ser245 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CREB-2 CREB2 TAXREB67 TXREB ATF4 DNA-binding protein TAXREB67 cyclic AMP-responsive element-binding protein 2 cAMP-responsive element-binding protein 2 cAMP response element-binding protein 2 tax-responsive enhancer element-binding protein 67 cy |
|---|---|
| Target | ATF4 (Phospho Ser245) |
| Molecular Weight(MW) | 45 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4 (Phospho-Ser245) Antibody (left) or the same antibody preincubated with blocking peptide (right)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
