Application
Various whole cell and tissue extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.| Product Name | ATG14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATG14 (autophagy related 14)Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATG14. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATG14 antibody ATG14 antibody ATG14L antibody BARKOR antibody KIAA0831 antibody autophagy related 14 antibody |
|---|---|
| Target | ATG14 |
| Molecular Weight(MW) | 55 kDa (note) |
Application
Various whole cell and tissue extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATG14 antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.| Positive Control | 293T , RD , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
