Application
Western blot analysi s of Apg5/Atg5 in Jurkat cell lysate.| Product Name | ATG5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATG5Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. An ubiquitin like conjugation system is involve in the formation of autophagosome where by Atg12 is covanletly bound to Apg5/ATG5. The conjugation is mediated by the ubiquitin-E1-like enzyme Atg7 and thE2-like enzyme Atg10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~37 kDa Apg5/Atg5 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.: |
|
| Species Reactivity | HumanMousePigRatBovine |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Autophagy protein5 atg5 APG5-like apg-5 autophagy protein Autophagy protein 5 Apoptosis-specific protein apg5 atg-5 APG5like Apoptosisspecific protein apg 5 atg 5 APG5 like Apoptosis specific protein |
|---|---|
| Target | ATG5 |
| Tissue Specificity | This antibody is specific for Apg5/Atg5. |
Application
Western blot analysi s of Apg5/Atg5 in Jurkat cell lysate.| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~37 kDa Apg5/Atg5 from samples of human, mouse and rat origins. Reactivity to other species has not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use.Store at -20 °C. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal |
