Application
Immunohistochemistry: ATG5 Antibody [ - Analysis of ATG5 in mouse intestine using DAB with hematoxylin counterstain.Application
Immunocytochemistry/Immunofluorescence: Immunofluorescent staining of SY5Y cells using at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator. Immunofluorescent staining of SY5Y cells using at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator.Application
Immunohistochemistry: Staining of hepatocytes using at 2.5ug/ml. Human liver hepatocytes 40X magnification.Application
Western Blot: Detection of ATG5 in mouse wildtype ES cell lysate (Lane 1) using Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center'| Product Name | ATG5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to ATG5Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to A |
| Immunogen | A synthetic peptide made to an N-terminal region (within residues 1-50) of the human ATG5 protein. [Swiss-Prot# Q9H1Y0] |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| immunohistochemistry 2.5ug/ml, immunofluorescence , Western Blot 1:500: |
|
| Species Reactivity | HumanMousePigRatZebra FishPrimateXenopusBovine |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Autophagy protein5 APG5-like Autophagy protein 5 APG5like APG5 like |
|---|---|
| Target | ATG5 |
| Tissue Specificity | This is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275. |
| Cellular Localization | Cytoplasm. Co-localizes with non-muscle actin. |
Application
Immunohistochemistry: ATG5 Antibody [ - Analysis of ATG5 in mouse intestine using DAB with hematoxylin counterstain.
Application
Immunocytochemistry/Immunofluorescence: Immunofluorescent staining of SY5Y cells using at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator. Immunofluorescent staining of SY5Y cells using at 1:250. Incubated overnight at 4 degrees. Photo courtesy of an anonymous collaborator.
Application
Immunohistochemistry: Staining of hepatocytes using at 2.5ug/ml. Human liver hepatocytes 40X magnification.
Application
Western Blot: Detection of ATG5 in mouse wildtype ES cell lysate (Lane 1) using Lane 2 is a mouse ATG5 KO ES cell lysate (negative control). Atg5-/- ES cells from Dr. Noboru Mizushima [Mizushima, N. et al. J. Cell Biol. 152 (2001)] Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center'| Application Notes | immunohistochemistry 2.5ug/ml, immunofluorescence , Western Blot 1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS plus 30% glycerol |
