Application
Immunofluorescence analysis of methanol-fixed HeLa, using ATP synthase B1 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ATP synthase B1 antibody detects ATP5F1 protein at cytosol on human colon by immunohistochemical analysis. Sample: Paraffin-embedded colon. ATP synthase B1 antibody dilution: 1:500.| Product Name | ATP synthase B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP synthase B1 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit B1)This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the b subunit of the proton channel. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP synthase B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (80%), Rhesus Monkey (89%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC24431 antibody PIG47 antibody ATP5F1 antibody H+-ATP synthase subunit b antibody ATP synthase subunit b mitochondrial antibody cell proliferation-inducing protein 47 antibody ATP synthase B chain mitochondrial antibody ATP synthase H+ transpor |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) |
| Cellular Localization | Mitochondrion , Mitochondrion inner membrane |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using ATP synthase B1 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ATP synthase B1 antibody detects ATP5F1 protein at cytosol on human colon by immunohistochemical analysis. Sample: Paraffin-embedded colon. ATP synthase B1 antibody dilution: 1:500.| Positive Control | Molt-4 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
