Rabbit Polyclonal antibody to ATP5HMitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]
Immunogen
Recombinant protein of human ATP5H
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IFIHC-PIPWB
WB 1:500 - 1:2000, IHC 1:20 - 1:200, IF 1:10 - 1:100, IP 1:20 - 1:50:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
ATP synthase H+ transporting mitochondrial Fo complex subunit d ATPQ
Target
ATP5H
Molecular Weight(MW)
18 kDa (note)
Application
Application
Western blot analysis of extract of various cells, using ATP5H antibody.
Application Notes
WB 1:500 - 1:2000, IHC 1:20 - 1:200, IF 1:10 - 1:100, IP 1:20 - 1:50: