Application
Immunoprecipitation of ATP Synthase beta from rat neuronal/glial cell extract.Application
Indirect immunofluorescence staining of rat neuronal/glial cell culture.Application
Immunoprecipitation of ATP Synthase beta in THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of ATP Synthase beta antibody [4.3E8.D10] overnight at 4°C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with ATP Synthase beta antibody [4.3E8.D10] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.| Product Name | ATP Synthase beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4.3E8.D10] to ATP Synthase betabeta subunit of rat liver ATP synthase [RGD, Feb 2006] |
| Immunogen | Intact rat mitochondria. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4.3E8.D10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| ICC/IF: 1:100 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | ATP synthase H+ transporting mitochondrial F1 complex beta polypeptide Atp5b |
|---|---|
| Target | ATP Synthase beta |
Application
Immunoprecipitation of ATP Synthase beta from rat neuronal/glial cell extract.
Application
Indirect immunofluorescence staining of rat neuronal/glial cell culture.
Application
Immunoprecipitation of ATP Synthase beta in THP-1 cells. Antigen-antibody complexes were formed by incubating 500ug of THP-1 whole cell lysate (in 500ul volume) with 5ul of ATP Synthase beta antibody [4.3E8.D10] overnight at 4°C. The immune complexes were captured on 30ul of protein G agarose, washed extensively, and eluted. Samples were resolved on an 8% SDS-PAGE gel, transferred to a PVDF membrane, and blocked with 5% milk in TBST for 1 hour at room temperature. The membrane was probed with ATP Synthase beta antibody [4.3E8.D10] at a dilution of 1:1000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.| Application Notes | ICC/IF: 1:100 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide and 1 mg/ml BSA |
