Application
ATP synthase gamma antibody detects ATP synthase gamma protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ATP synthase gamma antibody dilution: 1:500.Application
ATP synthase gamma antibody detects ATP synthase gamma protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. ATP synthase gamma antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: HeLa 12% SDS PAGE ATP synthase gamma(mitochondrial) antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ATP synthase gamma antibody detects ATP5C1 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 12% SDS-PAGE ATP synthase gamma antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ATP synthase gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP synthase gamma (ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1)This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the gamma subunit of the catalytic core. Alternatively spliced transcript variants encoding different isoforms have been identified. This gene also has a pseudogene on chromosome 14. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP synthase gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATP5C antibody ATP5CL1 antibody ATP5C1 antibody F-ATPase gamma subunit antibody mitochondrial ATP synthase gamma subunit 1 antibody ATP synthase subunit gamma mitochondrial antibody ATP synthase gamma chain mitochondrial antibody ATP synthase H+ |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (4) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:26991006) |
| Cellular Localization | Mitochondrion , Mitochondrion inner membrane |
Application
ATP synthase gamma antibody detects ATP synthase gamma protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ATP synthase gamma antibody dilution: 1:500.
Application
ATP synthase gamma antibody detects ATP synthase gamma protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. ATP synthase gamma antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: HeLa 12% SDS PAGE ATP synthase gamma(mitochondrial) antibody diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ATP synthase gamma antibody detects ATP5C1 protein by western blot analysis. A. 50 µg rat kidney lysate/extract 12% SDS-PAGE ATP synthase gamma antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , mouse brain , rat kidney |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
