Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200.Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at membrane on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500.Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500.Application
ATP5A1 antibody detects ATP5A1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.| Product Name | ATP5A1 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP5A1 (ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle)This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human ATP5A1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, Chimpanzee, BovineZebrafish (90%), Xenopus laevis (89%), Dog (95%), Pig (96%), Chicken (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATP5A antibody ATP5AL2 antibody ATPM antibody MOM2 antibody OMR antibody ORM antibody hATP1 antibody ATP5A1 antibody ATP synthase subunit alpha mitochondrial antibody mitochondrial ATP synthetase oligomycin-resistant antibody ATP synthase H+ t |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (5) |
| Cellular Localization | Mitochondrion inner membrane |
Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. ATP5A1 antibody [C2C3], C-term dilution: 1:200.
Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: ATP5A1 protein stained by ATP5A1 antibody [C2C3], C-term diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at membrane on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. ATP5A1 antibody [C2C3], C-term dilution: 1:500.
Application
ATP5A1 antibody [C2C3], C-term detects ATP5A1 protein at cytoplasm on mouse stomach by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ATP5A1 antibody [C2C3], C-term dilution: 1:500.
Application
ATP5A1 antibody detects ATP5A1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody diluted by 1:2000.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP5A1 antibody [C2C3], C-term diluted at 1:1000.| Positive Control | K562 , THP-1 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
