Application
Sample(30 ug whole cell lysate) A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE diluted at 1:500Application
ATP synthase delta antibody detects ATP5D protein at mitochondria on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. ATP synthase delta antibody dilution: 1:250.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000| Product Name | ATP5D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATP5D (ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit)This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP5D. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (89%), Rhesus Monkey (98%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATP5D antibody ATP synthase subunit delta mitochondrial antibody mitochondrial ATP synthase delta subunit antibody mitochondrial ATP synthase complex delta-subunit precusor antibody F-ATPase delta subunit antibody ATP synthase H+ transporting mito |
|---|---|
| Molecular Weight(MW) | 17 kDa (note) (2) |
| Cellular Localization | Mitochondrion , Mitochondrion inner membrane |
Application
Sample(30 ug whole cell lysate) A:Hep G2 B:MOLT4 C:Raji 12% SDS PAGE diluted at 1:500
Application
ATP synthase delta antibody detects ATP5D protein at mitochondria on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. ATP synthase delta antibody dilution: 1:250.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000| Positive Control | HepG2 , Molt-4 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
