Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT811] diluted at 1:500.Application
ATP6V1A antibody [GT811] detects ATP6V1A protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATP6V1A protein stained by ATP6V1A antibody [GT811] diluted at 1:200. Blue: Hoechst 33342 staining.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT811] diluted at 1:1000.| Product Name | ATP6V1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT811] to ATP6V1A (ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A)This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT811 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (96%), Rat (95%), Xenopus laevis (90%), Pig (96%), Chicken (83%), Rhesus Monkey (99%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATP6A1 antibody ATP6V1A antibody [GT811] antibody ATP6V1A antibody ATP6V1A1 antibody ATPase H+ transporting V1 subunit A antibody HO68 antibody V-ATPase subunit A antibody VA68 antibody VPP2 antibody Vma1 antibody |
|---|---|
| Target | ATP6V1A |
| Molecular Weight(MW) | 68 kDa (note) |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT811] diluted at 1:500.
Application
ATP6V1A antibody [GT811] detects ATP6V1A protein at cytoplasm by immunofluorescent analysis. Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATP6V1A protein stained by ATP6V1A antibody [GT811] diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT811] diluted at 1:1000.| Positive Control | 293T , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
