Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. ATR (phospho Thr1989) antibody diluted at 1:250.Application
ATR (phospho Thr1989) antibody detects ATR protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ATR (phospho Thr1989) antibody diluted at 1:250.Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR (phospho Thr1989) protein stained by ATR (phospho Thr1989) antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) antibody diluted at 1:200.Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) antibody diluted at 1:200.Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. ATR (phospho Thr1989) antibody diluted at 1:250.Application
Immunoprecipitation of ATR (phospho Thr1989) protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 μg of ATR (phospho Thr1989) antibody . Western blot analysis was performed using ATR (phospho Thr1989) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. ATR (phospho Thr1989) antibody diluted at 1:250.Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ATR (phospho Thr1989) antibody diluted at 1:250.Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody diluted at 1:1000.Application
Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | ATR (phospho Thr1989) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to ATR (phospho Thr1989) (ataxia telangiectasia and Rad3 related)The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq] |
| Modification | p-Thr1989 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FRP1 antibody MEC1 antibody SCKL antibody SCKL1 antibody ATR antibody FRAP-related protein 1 antibody serine/threonine-protein kinase ATR antibody ataxia telangiectasia and Rad3-related protein antibody Rad3 related protein antibody FRAP-related p |
|---|---|
| Molecular Weight(MW) | 301 kDa (note) |
| Cellular Localization | Nucleus |
Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. ATR (phospho Thr1989) antibody diluted at 1:250.
Application
ATR (phospho Thr1989) antibody detects ATR protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ATR (phospho Thr1989) antibody diluted at 1:250.
Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATR (phospho Thr1989) protein stained by ATR (phospho Thr1989) antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50. Scale bar = 10 μm.
Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ATR (phospho Thr1989) antibody diluted at 1:200.
Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ATR (phospho Thr1989) antibody diluted at 1:200.
Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human lung by immunohistochemical analysis. Sample: Paraffin-embedded human lung. ATR (phospho Thr1989) antibody diluted at 1:250.
Application
Immunoprecipitation of ATR (phospho Thr1989) protein from HCT-116 whole cell extracts treated with Hydroxyurea for 6 hr using 5 μg of ATR (phospho Thr1989) antibody . Western blot analysis was performed using ATR (phospho Thr1989) antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. ATR (phospho Thr1989) antibody diluted at 1:250.
Application
ATR (phospho Thr1989) antibody detects ATR (phospho Thr1989) protein at nucleus in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ATR (phospho Thr1989) antibody diluted at 1:250.
Application
Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody diluted at 1:1000.
Application
Raji whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR (phospho Thr1989) antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Raji nuclear extract , HCT116 , HCT116 100 J/m2 UVC treatment and recover for 6 hr |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
