Application
Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 g) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure.| Product Name | ATR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to ATRAtaxia Telangiectasia Mutated (ATM) and Rad 3-related protein (ATR) is a phosphatidylinositol kinase (PK)-related kinase which functions in response to DNA damage and repair as well as at DNA replication checkpoints during the cell cycle. ATR is a member of the DNA-PK kinases closely related to ATM and DNA-PK for which DNA stimulates the observed kinase activity. Chromosomal remodeling proteins have also been reported to associate with ATR complexes. Several known components of the NuRD complex including histone deacetylase 1 (HDAC1), HDAC2, and CHD4. |
| Immunogen | Immunogen was a synthetic peptide, which represented a portion of human Ataxia and Rad3 related encoded within exon 5 (LocusLink ID 545). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of .033 - .067μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | SCKL1 Q13535 ATR 210600 MEC1 FRP1 SCKL 545 |
|---|---|
| Target | ATR |
| Tissue Specificity | ATR |
| Cellular Localization | Type I membrane protein (Probable). |
Application
Detection of human ATR by Western Blot. Samples: Whole cell lysate (20 g) from HeLa and U2OS cells separated on a 3 to 8% tris-acetate gel. Antibody: ab10312 used at 0.07 mg/ml. Detection: Chemiluminescence with 15 second exposure.| Positive Control | Whole cell lysate from HeLa and U2OS cells |
|---|---|
| Application Notes | For WB: Use at a dilution of .033 - .067μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 0.1ml Tris-citrate/phosphate. pH 7-8, with 0.1% sodium azide |
