Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);Application
Flow cytometric analysis of K562 cells using AURKA mouse mAb (green) and negative control (purple).Application
Immunofluorescence analysis of Hela cells using AURKA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Application
Western blot analysis using AURKA mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.| Product Name | Aurora A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal Antibody [1F8] to Aurora AThe protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human AURKA expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F8 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | PPP1R47 STK7 AURA ARK1 BTAK STK6 AIK STK15 AURORA2 |
|---|---|
| Target | AURKA |
| Molecular Weight(MW) | 48 kDa (note) |
Application
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Application
Flow cytometric analysis of K562 cells using AURKA mouse mAb (green) and negative control (purple).
Application
Immunofluorescence analysis of Hela cells using AURKA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Application
Western blot analysis using AURKA mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
