Mouse monoclonal antibody [35C1] to Aurora AThe protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Immunogen
Recombinant Aurora A
Key Feature
Clonality
Monoclonal
Isotype
IgG2
Clone Number
35C1
Host Species
Mouse
Tested Applications
ICC/IFIHCIPWBPLA
Western Blotting: 1/500 - 1/1000 Optimal dilution / concentration should be determined by the end user.:
Species Reactivity
HumanMouse
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
STK15 STK7 AURKA AURA AIK aurora kinase A BTAK AURORA2 ARK1 PPP1R47 STK6
Target
Aurora A
Tissue Specificity
This antibody recognizes an epitope within the non-catalytic N-terminal domain of Aurora A. Clone 35C1 does not inhibit Aurora A kinase activity (Cremet et al. 2003).
Application
Application
WB analysis of Aurora A kinase recombinant protein using Aurora A antibody
Application Notes
Western Blotting: 1/500 - 1/1000 Optimal dilution / concentration should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.