Application
'Western blot analysis of Bassoon Lane 1: MW marker Lane 2: Rat Brain Tissue Lysate'Application
Immunohistochemistry analysis of bassoon localization in the rat brain cerebellar molecular layer using Bassoon, mAb (SAP7F407)| Product Name | Bassoon antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [SAP7F407] to BassoonBassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors.Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release. |
| Immunogen | Recombinant rat bassoon fragment (residues 738-1035) expressed asa fusion protein in E.coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | SAP7F407 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIPWB |
| ICC: Use at a concentration of 0.5 - 5 μg/ml. IHC: Use at a concentration of 2 μg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 400 kDa (predicted molecular weight: 472.6 kDa). Additional bands between 97 and 400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | Bsn 29138 Bassoon |
|---|---|
| Target | Bassoon |
Application
'Western blot analysis of Bassoon Lane 1: MW marker Lane 2: Rat Brain Tissue Lysate'
Application
Immunohistochemistry analysis of bassoon localization in the rat brain cerebellar molecular layer using Bassoon, mAb (SAP7F407)| Application Notes | ICC: Use at a concentration of 0.5 - 5 μg/ml. IHC: Use at a concentration of 2 μg/ml. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 400 kDa (predicted molecular weight: 472.6 kDa). Additional bands between 97 and 400 kDa are also identified and are reported to be proteolytic degradation products of Bassoon. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: 50% Glycerol, PBS, 0.1mM PMSF |
