Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-BAT1 mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAT1.Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BAT1 mouse monoclonal antibody. ( Dilution 1:50)Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BAT1 mouse monoclonal antibody. ( Dilution 1:50)| Product Name | BAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2C5] to BAT1Component of the THO subcomplex of the TREX complex. The TREX complex specifically associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism. Binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export. The recruitment occurs via an interaction between THOC4 and the cap-binding protein NCBP1. UAP56 functions as a bridge between THOC4 and the THO complex. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. The recruitment of the TREX complex to the intronless viral mRNA occurs via an interaction between KSHV ORF57 protein and THOC4. Splice factor that is required for the first ATP-dependent step in spliceosome assembly and for the interaction of U2 snRNP with the branchpoint. Has both RNA-stimulated ATP binding/hydrolysis activity and ATP-dependent RNA unwinding activity. Even with the stimulation of RNA, the ATPase activity is weak. Can only hydrolyze ATP but not other NTPs. The RNA stimulation of ATPase activity does not have a strong preference for the sequence and length of the RNA. However, ssRNA stimulates the ATPase activity much more strongly than dsRNA. Can unwind 5' or 3' overhangs or blunt end RNA duplexes in vitro. The ATPase and helicase activities are not influenced by U2AF2 and THOC4. |
| Immunogen | Full length human recombinant protein of human BAT1(NP_542165) produced in HEK293T cell. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 2C5 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| IHC 1:50,: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | D6S81E |
|---|---|
| Target | BAT1 |
| Tissue Specificity | is specific for human BAT1(NP_542165). |
Application
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-BAT1 mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAT1.
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BAT1 mouse monoclonal antibody. ( Dilution 1:50)
Application
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-BAT1 mouse monoclonal antibody. ( Dilution 1:50)| Application Notes | IHC 1:50,: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
