Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using UAP56 antibody at 1:100 dilution.Application
BAT1 antibody detects BAT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAT1 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
BAT1 antibody [N1C2] detects BAT1 protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. BAT1 antibody [N1C2] diluted at 1:500.Application
BAT1 antibody [N1C2] detects BAT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAT1 protein stained by BAT1 antibody [N1C2] diluted at 1:500. Red: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Scale bar = 10 μm.Application
UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. UAP56 antibody [N1C2] diluted at 1:500.Application
UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. UAP56 antibody [N1C2] diluted at 1:500.| Product Name | BAT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BAT1This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human UAP56. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Rhesus Monkey, Chimpanzee, BovineZebrafish (97%), Xenopus laevis (96%), Dog (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAT1 antibody D6S81E antibody UAP56 antibody DDX39B antibody nuclear RNA helicase (DEAD family) antibody 56 kDa U2AF65-associated protein antibody ATP-dependent RNA helicase p47 antibody spliceosome RNA helicase DDX39B antibody HLA-B-associated tra |
|---|---|
| Molecular Weight(MW) | 49 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using UAP56 antibody at 1:100 dilution.
Application
BAT1 antibody detects BAT1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAT1 antibody diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
BAT1 antibody [N1C2] detects BAT1 protein at nucleus in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. BAT1 antibody [N1C2] diluted at 1:500.
Application
BAT1 antibody [N1C2] detects BAT1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BAT1 protein stained by BAT1 antibody [N1C2] diluted at 1:500. Red: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Scale bar = 10 μm.
Application
UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. UAP56 antibody [N1C2] diluted at 1:500.
Application
UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. UAP56 antibody [N1C2] diluted at 1:500.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
