Application
MCF-7 activated with 1 μM Dexamethasone was separated on SDS-PAGE and probed with Monoclonal PA to BAX (MO) CL:2D2 . The antibody was developed with with Alkaline Phosphatase APA Mouse Fab ads HIgG conjugate and an NBT/BCIP substrate. Antibody dilution 5 μg/mL.| Product Name | Bax antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [2D2] to BaxBcl2 family is a key regulator of apoptosis that functions to either inhibit or promote cell death. Over-expression of members such as Bcl2 and BclxL inhibit the apoptotic process. The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacment of Bax. The displacement of Bax allows apoptosis to proceed. BclxS, a shorter version of BclxL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that BclxS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway. |
| Immunogen | Synthetic peptide: MDGSGEQPRGGGPTSS, corresponding to amino acids 3-16 of Human Bax. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2D2 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB: 1/10000. Predicted molecular weight: 21 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | HumanDogRatCatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P55269 600040 Bax 581 Bax zeta |
|---|---|
| Target | Bax |
| Cellular Localization | Cell Membrane |
Application
MCF-7 activated with 1 μM Dexamethasone was separated on SDS-PAGE and probed with Monoclonal PA to BAX (MO) CL:2D2 . The antibody was developed with with Alkaline Phosphatase APA Mouse Fab ads HIgG conjugate and an NBT/BCIP substrate. Antibody dilution 5 μg/mL.| Application Notes | WB: 1/10000. Predicted molecular weight: 21 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.08% Sodium Azide. Constituents: PBS |
