| Product Name | Bax antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [5B7] to BaxBcl-2, Bax, and Bcl-X are members of a family of proteins that regulate apoptosis. In contrast to Bcl-2Delta and Bcl-XL, overexpression of bax protein accelerates the programmed cell death (apoptosis). |
| Immunogen | A Synthetic peptide, aa 3-16 (Cys-GSGEQLGSGGPTSS), of mouse bax protein. Thisamino acid sequence is not shared by rat and human bax protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 5B7 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Immunofluorescence: assay dependent. Western Blotting: use at a concentration of 1μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 21 kDa, which corresponds to the predicted molecular weight of Bax.: |
|
| Species Reactivity | Mouse |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Bax 12028 |
|---|---|
| Target | Bax |
| Tissue Specificity | is raised against an amino acid sequence of mouse bax protein that is not shared byrat and human bax protein. |
| Cellular Localization | Cell Membrane |
| Light Chain Type | kappa |
| Positive Control | MAD109 or Schmidt-Rubin Cells |
|---|---|
| Application Notes | Immunofluorescence: assay dependent. Western Blotting: use at a concentration of 1μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 21 kDa, which corresponds to the predicted molecular weight of Bax.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
