| Product Name | BCA225 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CU18] to BCA225BCA 225 is a225 kDa glycoprotein secreted by the T47D breast carcinoma cell line. |
| Immunogen | BCA 225 protein secreted by the T47D (Clone 11) human breast carcinoma cellline. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CU18 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| Immunohistochemistry: use at a dilution of 1/40. Optimal dilutions / concentrations should be determined by the end user. Antigen retrieval is not essential, although may be beneficial. Strong intracytoplasmic staining is seen in primary and metastatic breast carcinomatissue, as well as in cervical carcinomas. Apical/glycocalyx staining isseen in normal kidney, lung, fallopian tube, liver, skin (eccrine sweat glands)and uterus. Similar staining patterns are observed in lung, ovarian, and endometrialcancers. Carcinomas of the colon, stomach, prostate, urinary bladder,liver, pancreas, thyroid, and parotid are negative, as are sarcomas and lymphoidcancers.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Target | BCA225 |
|---|---|
| Cellular Localization | Secreted |
| Positive Control | Breast carcinoma (human) |
|---|---|
| Application Notes | Immunohistochemistry: use at a dilution of 1/40. Optimal dilutions / concentrations should be determined by the end user. Antigen retrieval is not essential, although may be beneficial. Strong intracytoplasmic staining is seen in primary and metastatic breast carcinomatissue, as well as in cervical carcinomas. Apical/glycocalyx staining isseen in normal kidney, lung, fallopian tube, liver, skin (eccrine sweat glands)and uterus. Similar staining patterns are observed in lung, ovarian, and endometrialcancers. Carcinomas of the colon, stomach, prostate, urinary bladder,liver, pancreas, thyroid, and parotid are negative, as are sarcomas and lymphoidcancers.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS with 1% BSA and 0.1% sodium azide |
