Application
IHC-P image using - Detection of Bcl2 by IHC-P in human breast carcinoma tissue| Product Name | Bcl-2 (phospho Ser70) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bcl-2 (phospho Ser70)Bcl2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl2, such as in the case of translocation of Bcl2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C terminal ends. |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-S^P-P-L). |
| Modification | p-Ser70 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-P |
| ELISA: Use at a dilution of 1/10,000. IHC-P: Use at a dilution of 1/50-1/100. Detects a band of approximately 26 kDa (predicted molecular weight: 28.7 kDa). ELISA: positive result against immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bcl2 p Apoptosis regulator Bcl 2 Bcl 2 B cell CLL/lymphoma 2 BCL2 protein B cell leukemia/lymphoma 2 Bcl-2 phospho Ser70 Bcl2 phospho Ser70 Bcl2 phosphoS70 Apoptosis regulator Bcl2 Bcl-2 phosphoS70 Bcl phospho S70 Bcl2 phospho S70 B cell leuke |
|---|---|
| Target | Bcl-2 (phospho Ser70) |
| Cellular Localization | Endoplasmic reticulum, outer mitochondrial membrane and nuclear membrane. |
Application
IHC-P image using - Detection of Bcl2 by IHC-P in human breast carcinoma tissue| Positive Control | Breast carcinoma |
|---|---|
| Application Notes | ELISA: Use at a dilution of 1/10,000. IHC-P: Use at a dilution of 1/50-1/100. Detects a band of approximately 26 kDa (predicted molecular weight: 28.7 kDa). ELISA: positive result against immunizing peptide. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.3, 50% Glycerol and 150mM NaCl containing 0.02% sodium azide |
