Application
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Bcl-2 antibody [N1N2], N-term diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2 antibody at 1:250 dilution.Application
Bcl-2 antibody detects Bcl-2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human Bcl-2-transfected 293T cells 12% SDS PAGE Bcl-2 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Bcl-2 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BCL2 (B-cell CLL/lymphoma 2)This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, CatDog (100%), Cat (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Bcl-2 antibody BCL2 antibody protein phosphatase 1 regulatory subunit 50 antibody apoptosis regulator Bcl-2 antibody B-cell CLL/lymphoma 2 antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (2) |
| Cellular Localization | Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane |
Application
Bcl-2 antibody [N1N2], N-term detects Bcl-2 protein at cytoplasm in human squamous metaplasia of cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. Bcl-2 antibody [N1N2], N-term diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using Bcl-2 antibody at 1:250 dilution.
Application
Bcl-2 antibody detects Bcl-2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human Bcl-2-transfected 293T cells 12% SDS PAGE Bcl-2 antibody dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | human BCL2-transfected 293T , *PA-1 , *OKT3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
