Application
Bcl-X antibody detects BCL2L1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE Bcl-X antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A549, using BCL-x antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 μg of Bcl-X antibody . Western blot analysis was performed using Bcl-X antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Bcl-X antibody detects Bcl-X protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Bcl-X antibody diluted at 1:500.Application
Bcl-X antibody detects Bcl-X protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bcl-X protein stained by Bcl-X antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | Bcl-X antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bcl-X (BCL2-like 1)The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Bcl-X. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Dog, Cat, Pig, Rabbit, Sheep, Rhesus Monkey, BovineDog (100%), Cat (99%), Pig (97%), Rabbit (96%), Sheep (98%), Rhesus Monkey (99%), Bovine (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCL-XL/S antibody BCL2L antibody BCLX antibody BCLXL antibody BCLXS antibody Bcl-X antibody DKFZp781P2092 antibody bcl-xL antibody bcl-xS antibody BCL2L1 antibody apoptosis regulator Bcl-X antibody bcl-2-like protein 1 antibody protein phosphat |
|---|---|
| Molecular Weight(MW) | 26 kDa (note) (7) |
| Cellular Localization | Mitochondrion membrane; Single-pass membrane protein , Nucleus membrane; Single-pass membrane protein; Cytoplasmic side , Mitochondrion membrane , Nucleus membrane |
Application
Bcl-X antibody detects BCL2L1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 12% SDS-PAGE Bcl-X antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCL2L1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: HCT116 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A549, using BCL-x antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of Bcl-X protein from HCT-116 whole cell extracts using 5 μg of Bcl-X antibody . Western blot analysis was performed using Bcl-X antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Bcl-X antibody detects Bcl-X protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Bcl-X antibody diluted at 1:500.
Application
Bcl-X antibody detects Bcl-X protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bcl-X protein stained by Bcl-X antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Positive Control | HCT116 , NIH-3T3 , PC-12 , *OKT3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
