Application
Western Blot analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell lineApplication
Confocal microscopy of human β2-microglobulin-transfected canine cells labeled A) with Alexa Fluor®647 conjugate and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2µg/ml| Product Name | beta 2 Microglobulin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [B2M-01] to beta 2 MicroglobulinBeta 2 microglobulin plays a role in the assembly and surface expression of MHC class I molecules. It shows close homology in sequence to immunoglobulins suggesting a common evolutionary origin. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | B2M-01 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIPRIAWB |
| ELISA: Use at an assay dependant concentration. FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. RIA: Use at an assay dependant concentration. Dissociation constant of the antibody-soluble b2-microglobulin is 1.5 x 10^-8 mol/l as determined by competitive RIAWB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 109700 beta 2 Microglobulin beta2 Microglobulin 567 B2M P61769 |
|---|---|
| Target | beta 2 Microglobulin |
| Tissue Specificity | This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. |
| Cellular Localization | Secreted |
Application
Western Blot analysis (non-reducing conditions) of whole cell lysate of various cell lines using anti-human β2-microglobulin Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line
Application
Confocal microscopy of human β2-microglobulin-transfected canine cells labeled A) with Alexa Fluor®647 conjugate and B) with Alexa Fluor® 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2µg/ml| Application Notes | ELISA: Use at an assay dependant concentration. FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. RIA: Use at an assay dependant concentration. Dissociation constant of the antibody-soluble b2-microglobulin is 1.5 x 10^-8 mol/l as determined by competitive RIAWB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
