Application
Western blot analysis using anti beta-Actin mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12(5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.Application
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-beta Actin mAb (red, the secondary Ab is Cy3-Goat anti IgG). Blue: DRAQ5 fluorescent DNA dye.Application
Flow cytometric analysis of MCF-7 cells using anti-beta Actin mAb (right) and negative control (left).| Product Name | beta Actin antibody [8H10D10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [8H10D10] to beta ActinThis gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008] |
| Immunogen | The immunogen corresponding to human beta-Actin, conjugated to KLH. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 8H10D10 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HamsterHumanMonkeyMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ACTB , BRWS1 , PS1TP5BP1 |
|---|---|
| Target | beta Actin |
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Localized in cytoplasmic mRNP granules containing untranslated mRNAs. |
Application
Western blot analysis using anti beta-Actin mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12(5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Application
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using anti-beta Actin mAb (red, the secondary Ab is Cy3-Goat anti IgG). Blue: DRAQ5 fluorescent DNA dye.
Application
Flow cytometric analysis of MCF-7 cells using anti-beta Actin mAb (right) and negative control (left).| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
