Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg drosophila lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: beta Actin protein stained by beta Actin antibody [GT5512] diluted at 1:500. Blue: Hoechst 33342 staining.Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 10 µg yeast lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg mouse brain lysate/extract D. 30 µg PC-12 whole cell lysate/extract E. 30 µg rat brain lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg 30 hpf zebrafish lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. beta Actin antibody [GT5512] diluted at 1:250.Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract B. 30 µg MDA-MB-231 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody[GT5512] dilution: 1:20000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extract F. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:20000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg rabbit blood lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg goat blood lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Product Name | beta Actin antibody [GT5512] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT5512] to beta Actin (actin, beta)This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human beta Actin. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT5512 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseDrosophilaRatZebra FishYeastGoatRabbit |
| Predicted Reactivity | Japanese medaka, Xenopus laevis, Dog, Chicken, Sheep, Rhesus Monkey, Chimpanzee, Bovine, Guinea pigJapanese medaka (100%), Xenopus laevis (100%), Dog (100%), Chicken (100%), Sheep (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Molecular Weight(MW) | 42 kDa (note) |
|---|---|
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg drosophila lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody [GT5512] detects beta Actin protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in Triton X-100 for 1 min, then ice-cold methanol for 5 min. Green: beta Actin protein stained by beta Actin antibody [GT5512] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 10 µg yeast lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract C. 30 µg mouse brain lysate/extract D. 30 µg PC-12 whole cell lysate/extract E. 30 µg rat brain lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg 30 hpf zebrafish lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody [GT5512] detects beta Actin protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis. Sample: Paraffin-embedded rat adrenal gland. beta Actin antibody [GT5512] diluted at 1:250.
Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody[GT5512] detects beta Actin protein by western blot analysis. A. 30 µg MCF-7 whole cell lysate/extract B. 30 µg MDA-MB-231 whole cell lysate/extract 10% SDS-PAGE beta Actin antibody[GT5512] dilution: 1:20000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody [GT5512] diluted at 1:20000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extract F. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract 10% SDS-PAGE Beta Actin antibody [GT5512] dilution: 1:20000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg rabbit blood lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
beta Actin antibody [GT5512] detects beta Actin protein by western blot analysis. A. 30 µg goat blood lysate/extract 10% SDS-PAGE beta Actin antibody [GT5512] dilution: 1:10000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.| Positive Control | Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , NIH-3T3 , mouse brain , PC-12 , rat brain , drosophila , yeast , 30 hpf zebrafish , rabbit blood , MDA-MB-231 , goat blood , *L6 , *Ishikawa , *U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
