Application
Western blot Analysis. Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2μg/ml for 1 hour at 15-22ºC and developed by ECL (exp. time: 30 sec). Lane 1: bA4 (1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43)| Product Name | beta Amyloid antibody [BDI590], N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BDI590] to beta AmyloidThis gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq] |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | BDI590 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN2 |
|---|---|
| Target | beta Amyloid |
| Tissue Specificity | Amyloid, beta, N-terminal/APP |
| Cellular Localization | Cell surface protein that rapidly becomes internalized via clathrin-coated pits. During maturation, the immature APP (N-glycosylated in the endoplasmic reticulum) moves to the Golgi complex where complete maturation occurs (O-glycosylated and sulfated). A |
Application
Western blot Analysis. Amyloid beta A4 peptides were applied to SDS-PAGE and transferred to a PVDF membrane. The blot was probed with 2μg/ml for 1 hour at 15-22ºC and developed by ECL (exp. time: 30 sec). Lane 1: bA4 (1-40) Lane 2: bA4 (1-42) Lane 3: bA4 (1-43)| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at –20°C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
