| Product Name | beta Catenin (phospho Ser33) antibody [BC-76] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BC-76] to beta Catenin (phospho Ser33)The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. |
| Immunogen | Synthetic peptide, corresponding to amino acids 32-45 of Human beta Catenin, phosphorylated at Ser 33 . |
| Modification | p-Ser33 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgM |
| Clone Number | BC-76 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at a concentration of 5 μg/ml. WB: Use at a concentration of 5 μg/ml. Detects a band of approximately 94 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Unpurified |
| Gene Synonyms | CTNNB1, P35222, 114550, 1499, beta Catenin, CTNNB, beta Catenin (phospho S33), beta Catenin (phospho Ser33), beta Catenin (pS33), beta Catenin (pSer33), beta Catenin phospho S33, beta Catenin phospho Ser33, Phospho beta Catenin (S33), Phospho beta Catenin |
|---|---|
| Target | beta Catenin (phospho Ser33) |
| Tissue Specificity | Monoclonal Anti-Phospho-beta-Catenin (pSer 33 ) recognizes human beta-Catenin phosphorylated at Ser 33 . |
| Cellular Localization | Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). |
| Application Notes | ELISA: Use at a concentration of 5 μg/ml. WB: Use at a concentration of 5 μg/ml. Detects a band of approximately 94 kDa. Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
