| Product Name | beta Catenin (phospho Ser33/Ser37) antibody [BC-22] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BC-22] to beta Catenin (phospho Ser33/Ser37)The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. Absence of alpha-catenin is found in certain tumor cell lines and reduced levels in certain human carcinomas. Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. This interaction involves a 15-aa repeat in the APC. Beta-catenin cell levels seem to be controlled by APC. The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. |
| Immunogen | Synthetic peptide: DS p GIHS p GATTTAPS conjugated to KLH, corresponding to amino acids 32-45 of Human beta Catenin. |
| Modification | p-Ser33/Ser37 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | BC-22 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 5 - 10 μg/ml. Detects a band of approximately 94 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CTNNB1, P35222, 114550, 1499, beta Catenin, CTNNB, beta Catenin (phospho S33/S37), beta Catenin (phospho Ser33/Ser37), beta Catenin (pS33/pS37), beta Catenin (pSer33/pSer37), beta Catenin phospho S33/S37, beta Catenin phospho Ser33/Ser37, Phospho beta Cate |
|---|---|
| Target | beta Catenin (phospho Ser33/Ser37) |
| Tissue Specificity | Monoclonal Anti-phospho-beta-Catenin (pSer 33 /pSer 37 ) reacts specifically with beta-catenin phosphorylated at Ser 37 , and Ser 33 . At the peptide level, the antibody is inhibited more efficiently by the pSer 37 |
| Cellular Localization | Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). |
| Positive Control | Whole extract of cultured 293 (human embryonal kidney) cells treated with the proteasome inhibitor MG132. |
|---|---|
| Application Notes | ELISA: Use at a concentration of 5 - 10 μg/ml. WB: Use at a concentration of 5 - 10 μg/ml. Detects a band of approximately 94 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
