Application
Western blot analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody .Application
Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green) . Cell nuclei visualized by DAPI (blue).| Product Name | beta Catenin antibody [EM-22] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [EM-22] to beta CateninBeta-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound alpha, beta and gamma catenin. Beta-catenin links this complex through alpha-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumour cells. Beta-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 beta. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of beta-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.Entrez Gene: 3p21 |
| Immunogen | Recombinant human beta-catenin |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | EM-22 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIPWB |
| WB: Positive Control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line KW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line | |
| Species Reactivity | HumanMouseHamster |
| Concentration | 1mg/ml |
| Gene Synonyms | CTNNB, CTNNB1 |
|---|---|
| Target | beta-Catenin |
| Tissue Specificity | The antibody EM-22 reacts with C-terminal part of beta-catenin, an 88 kDa multifunctional protein involved both in cell adhesion and in activation of transcription. |
| Cellular Localization | Recruited to the nucleus upon ZNF639 overexpression. |
Application
Western blot analysis of beta-catenin in murine 3T3 (A), C57 (B) and KW1 (C) cell lines using EM-22 antibody .
Application
Immunocytochemistry staining of beta-catenin in human colon adenocarcinoma cell line HT29 using EM-22 antibody (green) . Cell nuclei visualized by DAPI (blue).| Application Notes | WB: Positive Control: HT29 human colon adenocarcinoma cell line, FHC human cell line, DLD1 human colon adenocarcinoma cell line KW1 mouse cell line C57MG mouse cell line, 3T3 mouse fibroblast cell line |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
