Application
beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody [GT2169] diluted at 1:200.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] diluted at 1:500.Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] diluted at 1:500.Application
Wild-type (WT) and beta Catenin knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Application
beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [GT2169] diluted at 1:200. Blue: Hoechst 33342 staining.| Product Name | beta Catenin antibody [GT2169] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT2169] to beta Catenin (catenin (cadherin-associated protein), beta 1, 88kDa)Beta-catenin is an adherens junction protein. Adherens junctions (AJs; also called the zonula adherens) are critical for the establishment and maintenance of epithelial layers, such as those lining organ surfaces. AJs mediate adhesion between cells, communicate a signal that neighboring cells are present, and anchor the actin cytoskeleton. In serving these roles, AJs regulate normal cell growth and behavior. At several stages of embryogenesis, wound healing, and tumor cell metastasis, cells form and leave epithelia. This process, which involves the disruption and reestablishment of epithelial cell-cell contacts, may be regulated by the disassembly and assembly of AJs. AJs may also function in the transmission of the 'contact inhibition' signal, which instructs cells to stop dividing once an epithelial sheet is complete.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human beta Catenin. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT2169 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Dog, Pig, Chicken, Sheep, Rhesus Monkey, BovineRat (100%), Zebrafish (95%), Xenopus laevis (97%), Dog (99%), Pig (99%), Chicken (97%), Sheep (99%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MRD19 , CTNNB , armadillo , catenin beta 1 , CTNNB1 |
|---|---|
| Target | beta Catenin |
| Molecular Weight(MW) | 85 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus , cytoskeleton |
Application
beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta Catenin antibody [GT2169] diluted at 1:200.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] diluted at 1:500.
Application
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] diluted at 1:500.
Application
Wild-type (WT) and beta Catenin knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with beta Catenin antibody [GT2169] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Application
beta Catenin antibody [GT2169] detects beta Catenin protein at cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta Catenin protein stained by beta Catenin antibody [GT2169] diluted at 1:200. Blue: Hoechst 33342 staining.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
