Application
ICC/IF analysis of SH-SY-5Y cells using beta III Tubulin antibody [SDL.3D10] at a dilution of 1:400 (red) and DAPI (blue).Application
Whole cell extract of rat brain cytosol was separated on SDS-PAGE and blotted with Monoclonal Anti-b-Tubulin. Isotype III . The antibody was developed with Goat anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lane A: Antibody dilution 1:400. Lane B: Negative control (only secondary antibody)Application
WB analysis of (1) U87, (2) MDBK and (3) RAT2 lysates using beta III Tubulin antibody [SDL.3D10] at a dilution of 1:400.| Product Name | beta Tubulin 3/ Tuj1 antibody [SDL.3D10] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [SDL.3D10] to beta III TubulinTubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar, but not identical, sets of alpha and beta tubulin polypeptides. Within either set of polypeptides, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the 15 residues of the carboxy-terminal. For beta-tubulin five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species, with the exception of the hematopoietic beta- tubulin which is the most highly divergent in sequence and is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. The monoclonal antibody from hybridoma SDL.3D10 can stimulate microtubule assembly when reconstituted with tubulin, tau or MAP2. |
| Immunogen | Chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype III conjugated to BSA. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | SDL.3D10 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWBIB |
| Indirect ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/400. Predicted molecular weight: 55 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 602661, TUBB4, Tubulin beta III, TUBB3, MC1R, Q13509, beta-4, beta4, 10381, beta 4 |
|---|---|
| Target | beta III Tubulin |
| Tissue Specificity | may be used for the localization of isotype III of beta-tubulin using various immunochemical assays. |
| Cellular Localization | Tubulin is the major constituent of microtubules. |
Application
ICC/IF analysis of SH-SY-5Y cells using beta III Tubulin antibody [SDL.3D10] at a dilution of 1:400 (red) and DAPI (blue).
Application
Whole cell extract of rat brain cytosol was separated on SDS-PAGE and blotted with Monoclonal Anti-b-Tubulin. Isotype III . The antibody was developed with Goat anti-Mouse IgG, Peroxidase conjugate and AEC substrate. Lane A: Antibody dilution 1:400. Lane B: Negative control (only secondary antibody)
Application
WB analysis of (1) U87, (2) MDBK and (3) RAT2 lysates using beta III Tubulin antibody [SDL.3D10] at a dilution of 1:400.| Positive Control | Rat brain extract. |
|---|---|
| Application Notes | Indirect ELISA: Use at an assay dependent dilution. WB: Use at a dilution of 1/400. Predicted molecular weight: 55 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Raw Ascites |
