| Product Name | beta Tubulin 4 antibody [ONS.1A6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [ONS.1A6] to beta Tubulin 4The major building block of microtubules is tubulin, an intracellular cylindrical filamentous structure that is present in almost all eukaryotic cells. Except in the simplest eukaryotes, tubulin exists in all cells as a 100 kD protein, a heterodimer of two similar but not identical polypeptides, designated alpha and beta, that assembles into microtubules. Both alpha and beta tubulins consist of various isotypes. For beta tubulin, six evolutionarily conserved isotypes were identified (designated beta I-beta VI). Beta IV exists as two subtypes in mammals, differing from each other at 10 positions. beta IVa is brain specific, whereas beta IVb is ubiquitous, and both appear to be constitutive. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis, organellar and flagellar movement, intracellular transport, and cytoskeletal functions. |
| Immunogen | Synthetic peptide corresponding to the C-terminal sequence, coupled to BSA. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | ONS.1A6 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. Use with 10% neutral buffered formalinalcoholic formalin-xylene, paraffin-embedded , or 1-2% glutaraldehyde-4% formaldehyde fixed, LR Gold embedded. WB: Use at a dilution of 1/400. Detects a band of approximately 55 kDa and possibly the 100 kDa dimer. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseRatHamsterBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 602661, TUBB4, TUBB3, MC1R, Tubulin beta IV, Q13509, beta-4, beta4, 10381, CFEOM3A, beta 4 |
|---|---|
| Target | Tubulin beta IV |
| Tissue Specificity | The antibody does not react with other tubulin isotypes. |
| Cellular Localization | Centrosomal |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. Use with 10% neutral buffered formalinalcoholic formalin-xylene, paraffin-embedded , or 1-2% glutaraldehyde-4% formaldehyde fixed, LR Gold embedded. WB: Use at a dilution of 1/400. Detects a band of approximately 55 kDa and possibly the 100 kDa dimer. Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: Ascites fluid. |
