| Product Name | beta Tubulin I/II antibody [JDR.3B8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [JDR.3B8] to beta Tubulin I/IITubulin is the major building block of microtubules. This intracellular, cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Except in the simplest eukaryotes, tubulin exists in all cells as a mixture of similar but not identical sets of alpha and beta tubulin polypeptides. Within either family, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxy-terminal 15 residues. For beta-tubulin, five evolutionarily conserved isotype clones have been identified. These are almost totally conserved in the subunits utilized in the same cell types of different species with the exception of the hematopoietic beta tubulin which is highly divergent in sequence and which is not conserved between species. Research has been centered around the hypothesis that these beta tubulin isotypes contribute to unique functional properties. It has been reported that the different isotypes of tubulin differ from each other in their ability to polymerize into microtubules. |
| Immunogen | A chemically synthesized peptide corresponding to the carboxyl-terminal sequence of human beta-tubulin isotype II coupled to BSA. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | JDR.3B8 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| IF: Use at an assay dependent dilution. The antibody localizes the beta-tubulin isotypes I and II in human and chicken fibroblasts. WB: Use at a dilution of 1/1000. The antibody localizes the tubulin band in a rat brain extract, bovine brain MAPs extract, chicken brain preparation, and chicken fibroblasts cell line. Predicted molecular weight: 55 kDa. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | HumanMouseChickenPigRatBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TUBB, TUBB2, dJ40E16.7 |
|---|---|
| Target | beta Tubulin I/II |
| Tissue Specificity | Using the immunoblotting technique, the antibody localizes the tubulin band in a rat brain extract, bovine brain MAPs extract, chicken brain preparation, and chicken fibroblasts cell line. Using the immunofluorescence technique, the antibody localizes the beta tubulin isotypes I and II in human and chicken fibroblasts. |
| Cellular Localization | Tubulin is the major constituent of microtubules. |
| Positive Control | Chicken fibroblasts extract, chicken brain tubulin preparation. |
|---|---|
| Application Notes | IF: Use at an assay dependent dilution. The antibody localizes the beta-tubulin isotypes I and II in human and chicken fibroblasts. WB: Use at a dilution of 1/1000. The antibody localizes the tubulin band in a rat brain extract, bovine brain MAPs extract, chicken brain preparation, and chicken fibroblasts cell line. Predicted molecular weight: 55 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: Raw Ascites |
