Application
The extracts of mouse liver(each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Product Name | BHMT antibody [3D6] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3D6 ] to BHMTBetaine homocysteine methyltransferase (BHMT), a cytosolic enzyme, and its partial fragments were discovered as autolysosomal membrane proteins from rat liver in the presence of leupeptin. BHMT was also found in human liver. BHMT transfers a methyl group from betaine to homocysteine to form DMG (dimethylglycine) and Met. In vivo, liver BHMT expression is influenced by dietary changes in sulphur amino acids, choline and betaine. |
| Immunogen | Recombinant human BHMT (1-406aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3D6 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1,000 ~ 2,000. Optimal working concentrations should be determined experimentally by the end user. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BHMT, Betaine homocysteine S methyltransferase 1, BHMT1, BHMT (Betainehomocysteine methyltransferase), Betainehomocysteine Smethyltransferase 1, EC2.1.1.5, EC 2.1.1.5, Betaine--homocysteine S-methyltransferase, Betainehomocysteine Smethyltransferase1, Beta |
|---|---|
| Target | BHMT |
| Light Chain Type | kappa |
Application
The extracts of mouse liver(each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.| Application Notes | Western Blot: Use at 1:1,000 ~ 2,000. Optimal working concentrations should be determined experimentally by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
