Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] diluted at 1:500.Application
BICD2 antibody [GT10811] detects BICD2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: BICD2 protein stained by BICD2 antibody [GT10811] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Immunoprecipitation of BICD2 protein from A431 whole cell extracts using 5 μg of BICD2 antibody [GT10811] . Western blot analysis was performed using BICD2 antibody [GT10811] . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
BICD2 antibody detects BICD2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody diluted by 1:1000.Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] diluted at 1:1000.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] diluted at 1:1000.| Product Name | BICD2 antibody [GT10811] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT10811] to BICD2 (bicaudal D homolog 2 (Drosophila))This gene is one of two human homologs of Drosophila bicaudal-D and a member of the Bicoid family. It has been implicated in dynein-mediated, minus end-directed motility along microtubules. It has also been reported to be a phosphorylation target of NIMA related kinase 8. Two alternative splice variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human BICD2. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | GT10811 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | bA526D8.1 , bicaudal D homolog 2 (Drosophila) |
|---|---|
| Target | BICD2 |
| Molecular Weight(MW) | 94 kDa (note) (2) |
| Cellular Localization | Golgi apparatus , Cytoplasm , cytoskeleton |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] diluted at 1:500.
Application
BICD2 antibody [GT10811] detects BICD2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: BICD2 protein stained by BICD2 antibody [GT10811] diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Immunoprecipitation of BICD2 protein from A431 whole cell extracts using 5 μg of BICD2 antibody [GT10811] . Western blot analysis was performed using BICD2 antibody [GT10811] . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
BICD2 antibody detects BICD2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody diluted by 1:1000.
Application
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] diluted at 1:1000.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] diluted at 1:1000.| Positive Control | Rat2 , 293T , A431 , HeLa , HepG2 , mouse lung |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
