Application
Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3] or Bid antibody [N1C3-2] . Western blot analysis was performed using Bid antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000Application
Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of BID protein by 4 μg Bid antibody [N1C3] 5 % SDS-PAGE The immunoprecipitated BID protein was detected by Bid antibody [N1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Product Name | Bid antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bid (BH3 interacting domain death agonist)This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Bid. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FP497 antibody, MGC15319 antibody, MGC42355 antibody, BID antibody, BH3-interacting domain death agonist antibody, apoptic death agonist antibody, BID isoform L(2) antibody, p22 BID antibody, Human BID coding sequence antibody, desmocollin type 4 antibody, |
|---|---|
| Target | Bid |
| Molecular Weight(MW) | 22 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Mitochondrion membrane , BH3-interacting domain death agonist p15: Mitochondrion membrane , BH3-interacting domain death agonist p13: Mitochondrion membrane , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Isoform 2: Mitochondrion membrane |
Application
Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3] or Bid antibody [N1C3-2] . Western blot analysis was performed using Bid antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000
Application
Bid antibody [N1C3] immunoprecipitates BID protein in IP experiments. IP samples: Jurkat whole cell extract A. 40 μg Jurkat whole cell extract B. Control with 4 μg of preimmune Rabbit IgG C. Immunoprecipitation of BID protein by 4 μg Bid antibody [N1C3] 5 % SDS-PAGE The immunoprecipitated BID protein was detected by Bid antibody [N1C3] diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]| Positive Control | A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
