Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] diluted at 1:1000.Application
Bid antibody [N1C3-2] detects BID protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: BID protein stained by Bid antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33343 staining.Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] diluted at 1:500.Application
Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3-2] or Bid antibody [N1C3] . Western blot analysis was performed using Bid antibody [N1C3-2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Bid antibody [N1C3-2] detects Bid protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg H1299 whole cell lysate/extract D. 30 µg HeLa whole cell lysate/extract E. 30 µg HepG2 whole cell lysate/extract F. 30 µg Molt-4 whole cell lysate/extract G. 30 µg Raji whole cell lysate/extract 7.5 % SDS-PAGE Bid antibody [N1C3-2] dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Bid antibody at 1:500 dilution.Application
Bid antibody [N1C3-2] detects Bid protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bid protein stained by Bid antibody [N1C3-2] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | Bid antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Bid (BH3 interacting domain death agonist)This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq] |
| Immunogen | Full length human Bid Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FP497 antibody, MGC15319 antibody, MGC42355 antibody, BID antibody, BH3-interacting domain death agonist antibody, apoptic death agonist antibody, BID isoform L(2) antibody, p22 BID antibody, Human BID coding sequence antibody, desmocollin type 4 antibody, |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Mitochondrion membrane , BH3-interacting domain death agonist p15: Mitochondrion membrane , BH3-interacting domain death agonist p13: Mitochondrion membrane , Isoform 1: Cytoplasm , Isoform 3: Cytoplasm , Isoform 2: Mitochondrion membrane |
Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] diluted at 1:1000.
Application
Bid antibody [N1C3-2] detects BID protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: BID protein stained by Bid antibody [N1C3-2] diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bid antibody [N1C3-2] diluted at 1:500.
Application
Immunoprecipitation of Bid protein from Jurkat whole cell extracts using 5 μg of Bid antibody [N1C3-2] or Bid antibody [N1C3] . Western blot analysis was performed using Bid antibody [N1C3-2] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Bid antibody [N1C3-2] detects Bid protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg H1299 whole cell lysate/extract D. 30 µg HeLa whole cell lysate/extract E. 30 µg HepG2 whole cell lysate/extract F. 30 µg Molt-4 whole cell lysate/extract G. 30 µg Raji whole cell lysate/extract 7.5 % SDS-PAGE Bid antibody [N1C3-2] dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Bid antibody at 1:500 dilution.
Application
Bid antibody [N1C3-2] detects Bid protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bid protein stained by Bid antibody [N1C3-2] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
