Application
Immunofluorescence staining of methanol-fixed Hela cells using BIM(Phospho-Ser69) AntibodyApplication
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM(Phospho-Ser69) Antibody ) or the same antibody preincubated with blocking peptide(right).| Product Name | Bim (phospho Ser69) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Bim (phospho Ser69)Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis |
| Immunogen | Peptide sequence around phosphorylation site of serine 69 (P-A-S(p)-P-G) derived from Human BIM. |
| Modification | p-Ser69 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-P |
| Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BOD, BimEL, BimL, BCL2L11, bcl2-L-11, bcl-2 interacting protein Bim, bcl-2-like protein 11, bcl-2-related ovarian death agonist, bcl-2 interacting mediator of cell death, BCL2-like 11 (apoptosis facilitator) |
|---|---|
| Target | Bim (phospho Ser69) |
| Molecular Weight(MW) | 23 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using BIM(Phospho-Ser69) Antibody
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM(Phospho-Ser69) Antibody ) or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
