Application
Western blot analysis of extracts from Hela cells using BIM Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM Antibody or the same antibody preincubated with blocking peptide(right).| Product Name | Bim antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to BimInduces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis. |
| Immunogen | Peptide sequence around aa.67~71 (P-A-S-P-G) derived from Human BIM. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BOD, BimEL, BimL, BCL2L11, bcl2-L-11, bcl-2 interacting protein Bim, bcl-2-like protein 11, bcl-2-related ovarian death agonist, bcl-2 interacting mediator of cell death, BCL2-like 11 (apoptosis facilitator) |
|---|---|
| Target | Bim |
| Molecular Weight(MW) | 23 (note) |
| Tissue Specificity | It should recognize BIM isoform 1,isoform2,isoform4,isoform7,isoform13 and isoform 14 based on sequence homology. |
Application
Western blot analysis of extracts from Hela cells using BIM Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BIM Antibody or the same antibody preincubated with blocking peptide(right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
