Application
Immunohistochemistry of Bim in human skin cancer cells with Bim Antibody (Cat. No. ) at 20 μg/mL.Application
Western blot analysis of Bim expression in human K562 (K) and A549 (A) cell lysates with Anti-Bim (Cat. No. ) at 2 μg/mL.| Product Name | Bim antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to BimMembers in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2homology 3 (BH3) domain is a potent death domain. BH3domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat (1,2). Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines(1,2). |
| Immunogen | Peptide corresponding to amino acids 22 to 40 of human origin (1). The sequence is identical to that of mouse anddiffers from that of rat by one amino acid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| For WB: Use at a dilution of 1:900 - 1:1,100 dilution. Optimal concentrations/dilutions should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BIM, BCL2L11, BOD, BimEL, O43521, 603827, BimL, BAM, 10018 |
|---|---|
| Target | Bim |
| Cellular Localization | Intracytoplasmic membranes |
Application
Immunohistochemistry of Bim in human skin cancer cells with Bim Antibody (Cat. No. ) at 20 μg/mL.
Application
Western blot analysis of Bim expression in human K562 (K) and A549 (A) cell lysates with Anti-Bim (Cat. No. ) at 2 μg/mL.| Application Notes | For WB: Use at a dilution of 1:900 - 1:1,100 dilution. Optimal concentrations/dilutions should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4 with 0.02% sodium azide as preservative. |
