Rabbit polyclonal to BimBim belongs to the Bcl2 family of proteins containing a Bcl2 homology domain 3 (BH3). It is proapoptotic and exerts its effects by interacting with prosurvival members of the Bcl2 family like Bcl2, BclxL and Bclw.
Immunogen
The immunogen is located within aa 1-50 of O43521 (Uniprot).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHC-P
IHC-P: Use at a dilution of 1/25. Perform enzymatic antigen retrieval before commencing with the IHC staining protocol, by boiling the tissue sections in 10mM citrate buffer,pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.
By sequence homology is predicted to cross react with all other BIM isoforms
Cellular Localization
Intracytoplasmic membranes
Application
Application
Human Placenta stained with anti-BIM/BOD antibody
Application
at 1:25 dilution, staining Bim in human placenta by IHC-P
Application Notes
IHC-P: Use at a dilution of 1/25. Perform enzymatic antigen retrieval before commencing with the IHC staining protocol, by boiling the tissue sections in 10mM citrate buffer,pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes. Optimal dilutions/concentrations should be determined by the end user.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.