Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.Application
Western blot analysis of (A) 5 and (B) 25 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.Application
Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 μg/mL.| Product Name | Bim antibody [1C2C8] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to Bim [1C2C8]Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines. |
| Immunogen | Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim. |
| Clonality | Monoclonal |
|---|---|
| Clone Number | 1C2C8 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFWB |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Bcl2related ovarian death gene, Bcl-2-related ovarian death gene, Bcl2like 11, Bcl-2-like 11, BOD, Bcl2like11, Bim (1C2C8): Bcl2 interacting protein, Bim (1C2C8): Bcl-2 interacting protein, Bcl 2 like 11 |
|---|---|
| Target | Bim |
| Tissue Specificity | This antibody is specific for Bim |
Application
Immunofluorescence of Bim in K562 cells with Bim antibody at 20 μg/mL.
Application
Western blot analysis of (A) 5 and (B) 25 ng of Bim recombinant protein with Bim antibody at 1 μg/mL.
Application
Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 μg/mL.| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
