Application
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [ - Blimp-1 expression by CD19+ (thick black line) or CD8+Application
Western Blot: Blimp-1 Antibody (3H2-E8) [ - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using 1881: murine pre-B cell lysate (negative control)Application
Immunocytochemistry/Immunofluorescence: Blimp-1 Antibody (3H2-E8) [ - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelledApplication
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [ - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total miceApplication
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [ - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells| Product Name | Blimp1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to Blimp 1 [clone 3H2-E8]Blimp-1 (B lymphocyte maturation protein-1) is a nuclear protein with five zinc fingers in its carboxy terminus. It is a transcriptional repressor that is both required and sufficient to trigger terminal differentiation of B lymphocytes and monocyte/macrophages. Blimp-1 is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. It has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression. |
| Immunogen | A fragment of the mouse Blimp-1 (residues 199-409). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3H2-E8 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCWB |
| Suggested working dilutions: * Western Blot - 1:1,000-1:5,000 Immunocytochemistry - (+) previous supernatant tested at 1:50-1:100 Immunofluorescence - (+) previous supernatant tested at 1:200 with tyramide amplification Immunohistochemistry - (+) previous supernatant tested at 1:200 with tyramide amplification *The investigator should determine the optimal working dilution for a specific application. *18-81 murine pre-B cells may be used as a negative control, in Western Blot, for this antibody. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Target | Blimp-1 |
|---|---|
| Tissue Specificity | This antibody is specific for the Blimp-1. |
| Cellular Localization | Nuclear |
Application
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [ - Blimp-1 expression by CD19+ (thick black line) or CD8+
Application
Western Blot: Blimp-1 Antibody (3H2-E8) [ - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using 1881: murine pre-B cell lysate (negative control)
Application
Immunocytochemistry/Immunofluorescence: Blimp-1 Antibody (3H2-E8) [ - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled
Application
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [ - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice
Application
Flow Cytometry: Blimp-1 Antibody (3H2-E8) [ - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells| Application Notes | Suggested working dilutions: * Western Blot - 1:1,000-1:5,000 Immunocytochemistry - (+) previous supernatant tested at 1:50-1:100 Immunofluorescence - (+) previous supernatant tested at 1:200 with tyramide amplification Immunohistochemistry - (+) previous supernatant tested at 1:200 with tyramide amplification *The investigator should determine the optimal working dilution for a specific application. *18-81 murine pre-B cells may be used as a negative control, in Western Blot, for this antibody. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees C. Long term (> 6 months) aliquot and store at -20 degrees C. Avoid repeated freeze thaw cycles. |
| Storage Buffer | 0.025 ml of Protein G purified mouse ascites [1.2 mg/ml] in PBS containing 0.1% Sodium Azide. |
