| Product Name | Blood Group A antigen antibody [9A] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [9A] to Blood Group A AntigenBlood-group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. |
| Immunogen | Cell preparation: A431 cells (human vulval squamous carcinoma cell line). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG3 |
| Clone Number | 9A |
| Host Species | Mouse |
| Tested Applications | ELISAIP |
| ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Target | Blood Group A antigen |
|---|---|
| Tissue Specificity | 9A is suitable for binding to the EGF receptor in A431 cells only. These cells have a glycosylation defect which reveals otherwise cryptic sugar structures. |
| Cellular Localization | Cell Membrane |
| Application Notes | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: None. Constituents: PBS |
