| Product Name | Blood Group Lewis a antibody [T174] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [T174] to Blood Group Lewis aLewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens. |
| Immunogen | SK-CO-10 colon cancer cell line (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | T174 |
| Host Species | Mouse |
| Tested Applications | HAIHC-P |
| IHC-P: Use at a dilution of 1/40 or greater, for Biotin based detection systems. For optimal staining the primary antibody should be incubated 20-60 minutes at room temperature. Antigen retrieval is not essential. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Target | Blood Group Lewis a |
|---|---|
| Tissue Specificity | This antibody detects Lewis A (Type 1 chain) antigen and belongs to the BG-5 group of antibodies, one of a series of well-characterized murine monoclonal antibodies. |
| Application Notes | IHC-P: Use at a dilution of 1/40 or greater, for Biotin based detection systems. For optimal staining the primary antibody should be incubated 20-60 minutes at room temperature. Antigen retrieval is not essential. Optimal dilutions/concentrations should be determined by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 1% BSA, PBS |
