Application
Sample (30 ug of whole cell lysate) A: K562 B: NCI-H929 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000Application
BLZF1 antibody detects BLZF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BLZF1 protein stained by BLZF1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.| Product Name | BLZF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BLZF1 (basic leucine zipper nuclear factor 1)Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BLZF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Chimpanzee, BovineRat (84%), Chimpanzee (99%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GOLGIN-45 antibody, JEM-1 antibody, JEM-1s antibody, JEM1 antibody, MGC22497 antibody, BLZF1 antibody, p45 basic leucine-zipper nuclear factor antibody, JEM-1short protein antibody, cytoplasmic protein antibody, basic leucine zipper nuclear factor 1 antibo |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (3) |
| Cellular Localization | Golgi apparatus lumen , Isoform 1: Nucleus , Isoform 2: Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: K562 B: NCI-H929 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000
Application
BLZF1 antibody detects BLZF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BLZF1 protein stained by BLZF1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
