Application
Each lane has 20 ug of protein extracts from the rat insulinoma cell line INS-1. The lanes have increasing glucose concentrations from (A) 40, (B) 100, and (C) 200 mg/dl. 6.5% SDS PAGE diluted at 1:500Application
BLZF1 antibody detects BLZF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: BLZF1 protein stained by BLZF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
BLZF1 antibody [N2C3] detects BLZF1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. BLZF1 antibody [N2C3] dilution: 1:500.Application
BLZF1 antibody [N2C3] detects BLZF1 protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. BLZF1 antibody [N2C3] dilution: 1:500.| Product Name | BLZF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to BLZF1 (basic leucine zipper nuclear factor 1)Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human BLZF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Chimpanzee, BovineChimpanzee (99%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GOLGIN-45 antibody, JEM-1 antibody, JEM-1s antibody, JEM1 antibody, MGC22497 antibody, BLZF1 antibody, p45 basic leucine-zipper nuclear factor antibody, JEM-1short protein antibody, cytoplasmic protein antibody, basic leucine zipper nuclear factor 1 antibo |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (2) |
| Cellular Localization | Golgi apparatus lumen , Isoform 1: Nucleus , Isoform 2: Cytoplasm |
Application
Each lane has 20 ug of protein extracts from the rat insulinoma cell line INS-1. The lanes have increasing glucose concentrations from (A) 40, (B) 100, and (C) 200 mg/dl. 6.5% SDS PAGE diluted at 1:500
Application
BLZF1 antibody detects BLZF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: BLZF1 protein stained by BLZF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
BLZF1 antibody [N2C3] detects BLZF1 protein at cytosol and nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. BLZF1 antibody [N2C3] dilution: 1:500.
Application
BLZF1 antibody [N2C3] detects BLZF1 protein at cytosol and nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. BLZF1 antibody [N2C3] dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
