| Product Name | BMP2 antibody [65529.111] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [65529.111] to BMP 2Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (Hogan 1996, Reddi 1998 and Francis-West et al. 1999). Mature BMPs are 30-38 kDa proteins that assume a TGF-beta -like cysteine knot configuration. Lovostatin increases bone formation by turning on the bmp-2 gene (Mundy et al. 1999). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (Macias et al. 1999, Lecanda et al. 1997). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (Hogan 1996, Dale and Wardle 1999). BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (Massague 1994, Derynck 1997, Attisano 1993). |
| Immunogen | Recombinant full length protein expressed in E. coli (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 65529.111 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| I-ELISA: Use at a concentration of 0.5 - 1 μg/ml to detect a limit of ~3 ng/well recombinant human BMP-2. WB: Use at a concentration of 1 - 2 μg/ml for human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively. Optimal dilutions/concentrations should be determined by the end user. | |
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 650, BMP2A, BMP2, P12643, 112261 |
|---|---|
| Target | BMP2 |
| Tissue Specificity | Monoclonal anti-BMP-2 recognizes recombinant human BMP-2 by ELISA and immunoblotting. The antibody shows approximately 25% cross-reactivity with recombinant human BMP-4. |
| Application Notes | I-ELISA: Use at a concentration of 0.5 - 1 μg/ml to detect a limit of ~3 ng/well recombinant human BMP-2. WB: Use at a concentration of 1 - 2 μg/ml for human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively. Optimal dilutions/concentrations should be determined by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH 7.4, with 5% trehalose. |
